不同施氮量对冬小麦产量、效益及土壤理化性状的影响

为探明豫北地区高肥力地块不同施氮量对冬小麦产量、经济效益及土壤理化性状的影响,在安阳市北关区壤质潮土上开展了冬小麦不同施氮量试验,设常规施磷钾肥（W1,CK）、常规施肥减施氮肥30%（W2）、常规施肥减施氮肥15%（W3）、常规施肥减施氮肥5%（W4）、常规施肥（W5）和常规施肥增施氮肥5%（W6）6个处理。结果表明,随着氮肥施用量的减少冬小麦主茎叶龄、次生根数、茎蘖数、株高及穗长等农艺性状呈下降趋势,以W6处理最佳。冬小麦产量随着施氮量的增加呈上升趋势,W4、W5、W6处理与W1处理相比增幅分别达48.7%、48.9%、49.1%,W4、W5、W6处理间差异不显著;但与W2、W3处理间差异达到显著水平。不同施氮量处理经济效益较W1处理均有不同程度增加,W4较W1处理净增效益5 869.80元/hm ²,较W5处理净增效益24.45元/hm ²。不同施氮量处理对土壤有机质、全氮、有效磷、速效钾、缓效钾含量和土壤容重有显著影响,但对pH影响不显著。土壤全氮含量随施氮量的增加呈上升趋势;W4处理土壤容重最低。综合分析,以常规施肥减施氮肥5%处理效果最佳。     

大麦籽粒淀粉和β-葡聚糖积累特性研究

为研究大麦籽粒支链淀粉、直链淀粉和β-葡聚糖积累特性,及淀粉各组分与β-葡聚糖的关系,以2个皮大麦、1个裸大麦和1个糯裸大麦为试验材料,测定花后7、14、21、28d淀粉各组分及β-葡聚糖含量。结果表明,甘啤6号淀粉各组分含量随灌浆推进均逐渐升高,在成熟期达到最大值;甘垦啤7号和甘垦6号均呈先升高后降低趋势,在花后21d有1个峰值。糯大麦C 2-1直链淀粉含量显著低于非糯大麦;支链淀粉含量显著高于非糯大麦,整个灌浆期呈先升高后降低趋势。β-葡聚糖含量均随灌浆时间延长逐渐升高,成熟期含量最高;整个灌浆期糯大麦C 2-1含量显著高于非糯大麦。相关分析表明,直链淀粉/支链淀粉比值与β-葡聚糖含量呈极显著负相关,可以将直链淀粉/支链淀粉比值作为高β-葡聚糖品种选育的一个指标。Logistic方程拟合发现,直链淀粉、β-葡聚糖最终积累量与积累起势与有效积累时间有关,支链淀粉最终积累量取决于最高积累速率和平均积累速率。     

基于电子商务平台的食用菌市场分析

信息时代背景下,电商网络已经无处不在,深入到了人们生活的方方面面,融入了生活的各个细节,影响着人们的生活习惯和生活方式。转变销售战略、拓展食用菌电子商务思路,是当前食用菌生产企业的当务之急。     

改性纤维素的吸附性能及应用研究进展

纤维素作为天然生物基材料来源广泛,纤维素的改性及其在吸附方面的应用一直作为研究的热点。笔者介绍了纤维素基本结构,综述了纤维素化学改性方法以及改性纤维素对水体污染物吸附能力的提高。重点从改性纤维素良好的吸附性、吸附机理以及对水体中金属离子和水体中氮磷的吸附性展开叙述。提出了改性纤维素可应用于土壤当中,以期为土壤改良、作物高产提供全面的科学解释;同时改性纤维素可与肥料相结合,提高肥料利用率减缓因施肥造成的面源污染等问题。     

Effect of electroacupuncture on microglia phenotype in injured abducens nerve

AIM: To investigate the effect of electroacupuncture treatment on microglia phenotype in Beagle dogs with abducens nerve injury. METHODS: Healthy adult Beagle dogs were randomly divided into 4 groups:normal control group, sham operation group, injury group and electroacupuncture treatment group, with 5 dogs in each group. In sham operation group, only the isolated nerves were exposed. In injury group, the dogs were restrained when electroacupuncture was performed in electroacupuncture treatment group. In electroacupuncture treatment group, electroacupuncture was performed on the base of dog nerve injury. The experiment was continued for 2 weeks. Western blot was used to detect the expression levels of M1 and M2 markers in the microglia. RESULTS: The expression of inducible nitric oxide synthase (iNOS) in injury group was significantly higher than that in control group (P<0.05). In electroacupuncture treatment group, the expression of iNOS was significantly lower than that in injury group (P<0.05). The expression of interleukin-1β (IL-1β) in injury group and electroacupuncture treatment group was higher than that in control group and that in electroacupuncture treatment group was significantly lower than that in injury group (P<0.05). The expression of arginase in injury group and electroacupuncture treatment group was higher than that in control group (P<0.05), and that in electroacupuncture treatment group was higher than that in injury group (P<0.05). Meanwhile, the expression of brain-derived neurotrophic factor (BDNF) in injury group and electroacupuncture treatment group was increased compared with control group (P<0.05). But the expression of BDNF in electroacupuncture treatment group was higher than that in injury group (P<0.05).CONCLUSION: After abducens nerve injury, electroacupuncture treatment reduces the expression levels of M1 marker proteins in microglia and increases the expression levels of M2 marker proteins.     

Effect of miRNA-33 on ABCA1/ABCG1 expression in RAW264.7 macrophage-derived foam cells after Hcy treatment

AIM:To study whether homocysteine (Hcy) inhibits the expression of ATP-binding cassette transporter A1 (ABCA1) and ATP-binding cassette transporter G1 (ABCG1) by microRNA-33 (miRNA-33) signaling, and reduces the efficiency of reverse cholesterol transport (RCT).METHODS:RAW264.7 macrophages were induced by oxidized low-density lipoprotein (ox-LDL) to establish foam cell model. Oil red O staining was used to determine whether the model was established successfully. miRNA-33 mimics and miRNA-33 inhibitor were transfected into the cells by Lipofectamine 2000, and the cells were exposed to Hcy at concentration of 5 mmol/L for 24 h. The intracellular lipid droplets were observed by Oil red O staining. The expression of ABCA1 and ABCG1 at mRNA and protein levels was determined by real-time PCR and Western blot. The cellular cholesterol content was analyzed by HPLC, and effluent rate of cholesterol was detected by the method of liquid scintillation counting.RESULTS:Compared with blank control group, the lipid content in miRNA-33 mimics group was increased, and the expression of ABCA1 and ABCG1 at mRNA and protein levels was decreased (P<0.05). The intracellular cholesterol content was increased gradually (P<0.05), and the cellular cholesterol efflux rate was gradually decreased (P<0.05) in miRNA-33 mimics group. Compared with blank control group, the testing results in miRNA-33 inhibitor group were the opposition of those in miRNA-33 mimics group (P<0.05). No diffe-rence of the above indexes among blank control group, miRNA-33 mimics-NC group and miRNA-33 inhibitor-NC group was observed.CONCLUSION:Hcy inhibits the mRNA and protein expression of ABCA1 and ABCG1 through miRNA-33 signaling, and reduces the efficiency of RCT in RAW264.7 macrophage-derived foam cells.     

Analysis and Assessment on Heavy Metal Contamination in Cotton Seeds of Hunan Province

[Objective] In order to guarantee the quality and safety of cotton products and to explore the feasibility of alternative planting, the contamination condition of the heavy metals in cotton seeds in Hunan province was investigated and evaluated. [Method] Fifty-six cotton seed samples, collected in 6 counties of Hunan province, were collected to determine 6 indicators including cadmium, lead, copper, chromium, mercury and inorganic arsenic contents. This was followed by pollution index calculation, correlation analysis, and cluster analysis. [Results] The range of comprehensive pollution indexes of Junshan district, Anxiang county, Nanxian county, Wangcheng and Dingcheng districts varied between 0.366 5–0.577 0, which belonged to a secure level. However, the single pollution indexes of Hengyang county are relatively high. The comprehensive pollution index of Hengyang county was 0.801 6, meaning a mild pollution level. [Conclusion] The pollution status of cadmium, lead, copper, chromium, mercury and arsenic in Hunan cottonseed samples are generally acceptable. Cotton could be a substitute for rice on contaminated soil.     

2个与HSP70基因连锁的微卫星座位与牛运输应激性状的关联分析

试验探讨了2个与HSP70基因连锁的微卫星座位与牛运输应激性状的关联分析。选择120头12月龄、体重250 kg左右的健康西门塔尔杂种肉牛进行运输应激试验，并根据牛基因组遗传图谱，选择23号染色体上与HSP70基因连锁的微卫星座位BMS468和BM1258检测其在西门塔尔杂种肉牛样本群体中的多态性，采用最小二乘拟合一般线性模型分析微卫星座位与运输应激性状部分指标之间的关联效应。结果显示：微卫星座位BMS468共检测到5个等位基因（128、134、140、146、154 bp），优势等位基因为128和134 bp，有效等位基因数（Ne）、多态信息含量（PIC）和遗传杂合度（He）分别为3.66、0.68和0.73，遗传多态性较高，关联分析显示与运输后7 d平均日增重和发病率显著相关，其中128/128 bp基因型个体的运输后7 d平均日增重最大（P < 0.05），134/128 bp基因型个体的发病率最低（P < 0.05）；微卫星座位BM1258座位共检测到6个等位基因（99、101、103、113、117、119 bp），优势基因为101 bp，Ne、PIC和He分别为4.30、0.73和0.77，遗传多态性较丰富，关联分析显示，其与发病率显著相关，其中99/99 bp基因型个体的发病率最低（P < 0.05）。综上所述，2个微卫星座位可作为牛运输应激性状的潜在遗传标记，为开展牛抗运输应激性状的标记辅助选择提供科学依据。     

Effect of beclin-1 gene silencing on TuAKe-injured gastric cancer SGC-7901 cells

AIM: To observe the effect of beclin-1 silencing by the technique of RNA interference on the injury of human gastric cancer SGC-7901 cell by Sheliugu extract (the extract from tuber of Amorphophallus konjac, TuAKe). METHODS: To knock down the expression of beclin-1 gene, SGC-7901 cells were transfected with lentiviral vector carrying beclin-1-shRNA. The beclin-1 gene knock-down and non-knock-down SGC-7901 cells were treated with TuAKe. The cell viability was analyzed by CKK-8 assay. The percentages of apoptotic cells were detected by flow cytometry. The expression of beclin-1 and LC3 was detected by Western blot. RESULTS: The beclin-1 gene silencing decreased the protein expression of beclin-1 and increased the protein expression of LC3 in the SGC-7901 cells, leading to the decrease in cell viability and the increase in apoptotic rate (P<0.05). TuAKe increased the protein expression of beclin-1 and LC3 in the SGC-7901 cells, and decreased the protein expression of LC3 in the SGC-7901 cells with beclin-1 gene silencing, thus inhibiting the cell viability and increasing the apoptotic rate (P<0.05). CONCLUSION: Beclin-1 gene silencing inhibits the activation of beclin-1-related signaling pathway in gastric cancer SGC-7901 cells, and aggravates the injury of cell viability induced by TuAKe.